An article Development and Verification of a Precolumn Derivatization LC-MS/MS Method for the Pharmacokinetic Study of Houttuynine of Houttuynia Essential Oil WOS:000644610800001 published article about DNPH DERIVATIZATION; CORDATA THUNB.; CARBONYL-COMPOUNDS; VOLATILE OIL; IN-VIVO; IDENTIFICATION; HOUTTUYFONATE; SODIUM in [Liu, Yuanyuan; Yang, Yanfang; Wang, Bangyuan; Wang, Renyun; Pang, Jianmei; Jiang, Yu; Liu, Yuling] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China; [Liu, Yuanyuan; Yang, Yanfang; Wang, Bangyuan; Wang, Renyun; Pang, Jianmei; Jiang, Yu; Liu, Yuling] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Beijing Key Lab Drug Delivery Technol & Novel For, 1 Xiannongtan St, Beijing 100050, Peoples R China in 2021.0, Cited 31.0. Quality Control of Benzophenone. The Name is Benzophenone. Through research, I have a further understanding and discovery of 119-61-9
Houttuynia essential oil (HEO) has excellent antiviral, anti-inflammatory, and other pharmacological effects, but the lack of effective analytical methods to quantify HEO in plasma has hindered its better clinical monitoring. Houttuynine (Hou) is one of the main active ingredients and quality control substances of HEO, so the pharmacokinetic study of HEO could be conducted by determining Hou blood concentration. Hou is active and not stable in plasma, which makes its blood concentration difficult to measure. In this work, a novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method for Hou determination in rat blood was established that involves Hou being derivatized with 2, 4-dinitrophenylhydrazine to form a stable compound to prevent degradation. Herein, p-Tolualdehyde-2,4-dinitrophenylphenylhydrazone was selected as an internal standard substance and the LC-MS/MS method was evaluated for selectivity, precision, accuracy, calibration limit, matrix effect, recovery, and stability. Good linearity (r(2) = 0.998) was reached in the range of 2-2000 ng/mL, and the lower limit of quantification of Hou was determined to be 2 ng/mL. The mean intra-assay accuracy ranged from 77.7% to 115.6%, whereas the intra-assay precision (relative standard deviation, RSD) was below 11.42%. The matrix effect value for Hou in rat plasma was greater than 75%, and for the internal standard (IS) it was 104.56% +/- 3.62%. The extraction recovery of Hou were no less than 90%, and for the IS it was 96.50% +/- 4.68%. Our method is sensitive and reliable and has been successfully applied to the pharmacokinetic analysis of Hou in rats given HEO via gavage and injection.
Quality Control of Benzophenone. About Benzophenone, If you have any questions, you can contact Liu, YY; Yang, YF; Wang, BY; Wang, RY; Pang, JM; Jiang, Y; Liu, YL or concate me.
Reference:
Thiomorpholine – Wikipedia,
,Thiomorpholine | C4H9NS – PubChem