I found the field of Biochemistry & Molecular Biology; Chemistry very interesting. Saw the article Reduction of the extra-column band dispersion by a slow transport and splitting of a sample band in isocratic reversed-phase liquid chromatography published in 2021.0. COA of Formula: C13H10O, Reprint Addresses Fukusaki, E; Tanaka, N (corresponding author), Osaka Univ, Grad Sch Engn, Dept Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan.. The CAS is 119-61-9. Through research, I have a further understanding and discovery of Benzophenone
Sample introduction method was studied to reduce the extra-column effect in reversed-phase HPLC. Slow transport of a sample band (SToSB) in the pre-column space followed by the introduction of the band into the column at a near-optimum flow rate resulted in larger plate counts for a 1.0 mmID, 5 cm long column as much as 1.4-1.6 times for solutes with a retention factor (k) of 0.5-1.8 compared to a conventional elution method. Further reduction of the extra-column effect was possible by orthogonally splitting the sample band (SplSB) by flow switching during its slow transport followed by the introduction of the leading part of the band into the column. In this case, increased plate counts of up to 2-3 times for solutes with k of 0.5-1.8 were observed for a 1.0 mmID, 5 cm column. The sample introduction method, SToSB in the injector and the pre-column tube of a few mu L, was found to reduce the extra-column band variance by 0.4-0.5 mu L-2 for an UHPLC system with the extra-column volume (V-extra) of ca. 4.6 mu L and the system variance (sigma(2)(extra)) of 1.1 mu L-2 at flow rate of 100 mu L/min, while SToSB and subsequent SplSB were found to be more effective, reducing sigma(2)(extra) by about 0.8 mu L-2. With an UHPLC instrument with V(extra )of about 10 mu L and sigma(2)(extra) of ca. 3.6 mu L-2 at flow rate of 300 mu L/min, 1.4-2.1 times as many plate counts were observed with SToSB and SplSB compared to the normal elution method for early-eluting solutes with k=0.25-1.7 for a column, 2.1 mmID, 5 cm long. With this UHPLC instrument, SToSB and/or SplSB resulted in the reduction of sigma(2)(extra) by 1.2-2.2 mu L-2. (C) 2021 Elsevier B.V. All rights reserved.
Welcome to talk about 119-61-9, If you have any questions, you can contact Terada, H; Kuroda, I; Uzu, H; Ohira, M; Yoshikawa, K; Furuno, M; Fukusaki, E; Tanaka, N or send Email.. COA of Formula: C13H10O
Reference:
Thiomorpholine – Wikipedia,
,Thiomorpholine | C4H9NS – PubChem