Category: thiomorpholine. Recently I am researching about HYDROPHILIC-INTERACTION CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; ALPHA(1)-ACID GLYCOPROTEIN; MASS-SPECTROMETRY; GLYCOSYLATION; OLIGOSACCHARIDES; IDENTIFICATION; PROCAINAMIDE; FUCOSYLATION; SEPARATION, Saw an article supported by the Chung-Ang University Graduate Research Scholarship in 2018. Published in ACADEMIC PRESS INC ELSEVIER SCIENCE in SAN DIEGO ,Authors: Kim, W; Kim, J; You, S; Do, J; Jang, Y; Kim, D; Lee, J; Ha, J; Kim, HH. The CAS is 88-68-6. Through research, I have a further understanding and discovery of 2-Aminobenzamide
Sialylation can influence the stability, half-life, and immunogenicity of glycoproteins, but sialylated N-glycans are known to be difficult to analyze. Human alpha1-acid glycoprotein (AGP) is reported to have glycans that consist of sialylated N-glycans. The N-glycan profiling of AGP is qualitatively and quantitatively investigated here by UPLC and LC-ESI-MS/MS. Three fluorescent tags (AB, AA, and ProA) and two separation columns (HILIC and AEX-HILIC) were adopted to confirm and compare each analytical characteristic. The results of AA were comparable to those of the well-established AB. The qualification of ProA was notable due to its superior fluorescence intensity and ionization efficiency, and ProA showed smaller quantitative or larger-sized fragments in LC-ESI-MS/MS compared to AB and AA. However, the MS quantification of ProA was distorted because the increased sialylation level decreased the LC-ESI-MS/MS ionization efficiency. HILIC had better peak separability, AEX-HILIC had an advantage in UPLC sialylation profiling, and each isomeric glycan could be Identified by both columns in LC-ESI-MS/MS. In conclusion, ProA is favored for UPLC and LC-ESI-MS/MS detection but not reliable for MS quantification. This study firstly demonstrates the qualification and quantification of sialylated N-glycans by comparing the conunonly used analytical conditions with different fluorescent tags, columns, and instruments.
Welcome to talk about 88-68-6, If you have any questions, you can contact Kim, W; Kim, J; You, S; Do, J; Jang, Y; Kim, D; Lee, J; Ha, J; Kim, HH or send Email.. Category: thiomorpholine
Reference:
Thiomorpholine – Wikipedia,
,Thiomorpholine | C4H9NS – PubChem