Recently I am researching about 2,4-DICHLOROPHENOXYACETIC ACID; NUCLEOTIDE-SEQUENCE; INCP-1-BETA PLASMIDS; BACTERIAL CONSORTIUM; METABOLIC PATHWAY; DEGRADATION; RESISTANCE; TRANSPOSON; DIVERSITY; EVOLUTION, Saw an article supported by the Fonds voor Wetenschappelijk Onderzoek Vlaanderen (FWO)FWO [G.0371.06]; EU project METAEXPLORE (EU) [222625]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [Sonderforschungsbereich TRR 51]; High Performance and Cloud Computing Group at the Zentrum fur Datenverarbeitung of the University of Tubingen; Federal Ministry of Education and Research (BMBF)Federal Ministry of Education & Research (BMBF) [031 A535A]; Leibniz Open Access Publishing Fund [4055]. Published in OXFORD UNIV PRESS in OXFORD ,Authors: Ozturk, B; Werner, J; Meier-Kolthoff, JP; Bunk, B; Sproer, C; Springael, D. The CAS is 64-10-8. Through research, I have a further understanding and discovery of 1-Phenylurea. Recommanded Product: 1-Phenylurea
Biodegradation of the phenylurea herbicide linuron appears a specialization within a specific Glade of the Variovorax genus. The linuron catabolic ability is likely acquired by horizontal gene transfer but the mechanisms involved are not known. The full-genome sequences of six linuron-degrading Variovorax strains isolated from geographically distant locations were analyzed to acquire insight into the mechanisms of genetic adaptation toward linuron metabolism. Whole-genome sequence analysis confirmed the phylogenetic position of the linuron degraders in a separate Glade within Variovorax and indicated that they unlikely originate from a common ancestral linuron degrader. The linuron degraders differentiated from Variovorax strains that do not degrade linuron by the presence of multiple plasmids of 20-839 kb, including plasmids of unknown plasmid groups. The linuron catabolic gene clusters showed 1) high conservation and synteny and 2) strain-dependent distribution among the different plasmids. Most of them were bordered by IS1071 elements forming composite transposon structures, often in a multimeric array configuration, appointing IS1071 as a key element in the recruitment of linuron catabolic genes in Variovorax. Most of the strains carried at least one (catabolic) broad host range plasmid that might have been a second instrument for catabolic gene acquisition. We conclude that Glade 1 Variovorax strains, despite their different geographical origin, made use of a limited genetic repertoire regarding both catabolic functions and vehicles to acquire linuron biodegradation.
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Reference:
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