The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: 1-Methyl-4-nitro-1H-imidazol-5-amine( cas:4531-54-8 ) is researched.SDS of cas: 4531-54-8.Hiralben, S. Mehta; Shinghvi, Indrajeet; Raj, Hasumati A. published the article 《Development and validation of stability – indicating RP-HPLC chromatographic method by forced degradation studies for azathioprine by related substances》 about this compound( cas:4531-54-8 ) in International Journal of Pharmaceutical Sciences and Research. Keywords: azathioprine reverse phase high performance liquid chromatog. Let’s learn more about this compound (cas:4531-54-8).
Simple, rapid and reproducible stability-indicating methods were established for quant. determination of azathioprine using a, phenomenex 250 mm × 4.9 mm C18, 5 μm, inertsil and UV detection at 240 nm. The isocratic elution was used to quantify the analyte and the mobile phase was acetate buffer: acetonitrile: methanol (30: 35: 35) was pumped at 1.0 mL/min. The method was linear between 10-300 μg/mL, statistically validated for its linearity, precision and accuracy. In this study, degradation behavior of azathioprine was studied by subjecting the drug to various ICH stress conditions. The intra and inter day variation was found to be less than 1% showing high precision of the assay method. It was found that the excipients in the com. tablet did not interfere with the method. Developed method can routinely use for the estimation of azathioprine related compounds from the dosage form and also for stability sample.
Different reactions of this compound(1-Methyl-4-nitro-1H-imidazol-5-amine)SDS of cas: 4531-54-8 require different conditions, so the reaction conditions are very important.
Reference:
Thiomorpholine – Wikipedia,
Thiomorpholine | C4H9NS – PubChem