Zhang, Bi-cheng; Yang, Bo; Liu, Jian; Guan, Sha; Rao, Zhi-guo; Gao, Jian-fei published the article 《Phenotype identification of tumor-associated macrophages in mice bearing lung carcinoma》. Keywords: phenotype macrophage lung carcinoma.They researched the compound: 1-Iodo-2-methylbutane( cas:616-14-8 ).Name: 1-Iodo-2-methylbutane. Aromatic heterocyclic compounds can be divided into two categories: single heterocyclic and fused heterocyclic. In addition, there is a lot of other information about this compound (cas:616-14-8) here.
Objective To identify the phenotype of tumor-associated macrophages (TAM) in mice bearing Lewis lung carcinoma (LLC). Methods LLC cells were planted in the dorsal necks of C57BL/6 mice s.c. The levels of Th1/Th2 cytokines in the transplantation tumors were tested by ELISA. Co-expression of CD68/macrophage mannose receptor (MMR) and CD68/inducible nitric oxide synthase (iNOS) of TAM was detected by double-labeled immunofluorescence staining. Phagocytic capacity of TAM was assessed by yeast phagocytosis assay. Results In the mice LLC transplantation tumors, Th2 cytokine shift was found in the microenvironment. The concentrations of IFN-γ and IL-12 were (2.19 ±> 0.34) ng/mL and (1635.92 ±> 754.86) ng/mL in transplantation tumors, lower than (5.49 ±> 1.04) ng/mL and (6161.48 ±>498.49) ng/mL in normal lung tissues (P <0.05). IL-4 and IL-10 were (29.31 ±> 14.47) ng/mL and (21.54 ±> 10.72) ng/mL in transplantation tumors, higher than (16.43 ±>6.31) ng/mL and (10.71 ±>2.02) ng/mL in normal lung tissues (P <0.05). The percentage of CD68/MMR(+) TAM in all the TAM was 69.7%-83.2%, while CD68/iNOS (+) was 16.8%-30.3%. The phagocytic rate and index of the transplantation tumors were (5.42 ±> 1.74)% and 0.08 ±>0.02, much lower than (23.63 ±> 5.81)% and 0.39 ±>0.14 in normal spleen tissues with statistical significance (P <0.01). Conclusion TAM in mice bearing LLC might be polarized to the alternative activation phenotype. This compound(1-Iodo-2-methylbutane)Name: 1-Iodo-2-methylbutane was discussed at the molecular level, the effects of temperature and reaction time on the properties of the compound were discussed, and the optimum reaction conditions were selected.
Reference:
Thiomorpholine – Wikipedia,
Thiomorpholine | C4H9NS – PubChem